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Image Search Results
Journal: bioRxiv
Article Title: PTPN22 interacts with EB1 to regulate T cell receptor signaling
doi: 10.1101/481507
Figure Lengend Snippet: A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of CD25, CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet:
Techniques: Western Blot, Expressing, Transfection, Activity Assay
Journal: bioRxiv
Article Title: PTPN22 interacts with EB1 to regulate T cell receptor signaling
doi: 10.1101/481507
Figure Lengend Snippet: A, B Test the protein expression levels of CD25 and CD69 by use of ELISA. ELISA assays were used to test the expression levels of CD25 (A) or Same assays were used to test the expression levels of CD69 (B). C, D Test the mRNA transcription levels of CD25 and CD69 by use of qPCR. qPCR assays were used to test the expression levels of CD25 (C) or Same assays were used to test the expression levels of CD69 (D). Data information: Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet:
Techniques: Expressing, Enzyme-linked Immunosorbent Assay
Journal: Immunology
Article Title: T regulatory cells control T-cell proliferation partly by the release of soluble CD25 in patients with B-cell malignancies
doi: 10.1111/j.1365-2567.2010.03308.x
Figure Lengend Snippet: Soluble (s) CD25 is increased in chronic lymphocytic leukaemia (CLL) and lymphoma plasma. Plasma from CLL patients (n = 12), B-cell lymphoma patients (n = 14) and healthy controls (n = 27) was analysed for the presence of sCD25 using enzyme-linked immunosorbent assay. Patients displayed a significantly higher level of soluble CD25 than healthy individuals. Mann–Whitney U-test was used to calculate significant differences.
Article Snippet: Soluble CD25 enzyme-linked immunosorbent assay The presence of sCD25 was detected with CD25/IL-2 receptor enzyme-linked
Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY